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目的 通过对烹调烟雾职业暴露人群外周血免疫指标的测定了解烹调烟雾对机体免疫功能的影响。方法 对被试者 70人 ,其中暴露组 41人 ,对照组 2 9人。分别通过流式细胞仪直接荧光法、四甲基偶氮唑盐 (MTT)比色法、放射免疫分析法、生物素 -亲和素双抗体夹心酶联免疫吸附 (ABC- EL ISA)技术分析人外周血 T、B淋巴细胞及亚群、自然杀伤细胞 (NK细胞 )活性、白细胞介素 - 2 (IL- 2 )、γ-干扰素 (IFN- γ)水平。结果 经成组 t检验 ,暴露组 CD3+细胞百分率、NK细胞杀伤率明显低于对照组 (P<0 .0 1) ,工龄 7年以上被调查者中暴露组 CD4+ CD8- T细胞百分率、CD4+ / CD8+细胞比值明显低于对照组 (P<0 .0 5 ) ;而 CD8+ CD4- T、CD19+细胞百分率、IL- 2、IFN- γ水平两组间差异无统计学意义 (P>0 .0 5 )。结论 长期接触烹调烟雾对机体细胞免疫功能有一定抑制作用
Objective To understand the influence of cooking smoke on the immune function by measuring the peripheral blood immune parameters of occupationally exposed people in cooking smoke. Methods The subjects were 70 people, of which 41 were exposed group, control group 29 people. The cytotoxicity was detected by direct flow cytometry, MTT colorimetric assay, radioimmunoassay, and ABC-ELISA Peripheral blood T, B lymphocytes and subpopulations, natural killer (NK) cell activity, interleukin - 2 (IL - 2) and interferon - γ levels were measured. Results The percentages of CD3 + cells and NK cells in exposed group were significantly lower than those in control group (P <0.01). The percentages of CD4 + CD8 + T cells in CD4 + / CD8 + CD8 + cells were significantly lower than those in the control group (P <0.05). There was no significant difference between the two groups in the percentage of CD8 + CD4-T and CD19 + cells, IL-2 and IFN- γ (P> 0.05) ). Conclusion Long-term exposure to cooking smoke has a certain inhibitory effect on cellular immune function