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目的初步研究醋柳黄酮对大鼠非酒精性脂肪性肝病的疗效。方法雄性SD大鼠54只随机分组,正常对照组20只喂饲普通饲料,其余大鼠喂饲高脂饲料(88%标准普通饲料+10%猪油+2%胆固醇)。正常对照组和高脂饮食大鼠各10只于12周末处死观察造模效果。剩余高脂饮食大鼠24只分为模型对照组和醋柳黄酮治疗组。治疗组自13周开始进行醋柳黄酮灌胃治疗(10mg/d),于20周末处死各组大鼠,测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、γ-谷氨酰转肽酶(GGT)、碱性磷酸酶(ALP)、总蛋白(TP)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性,同时观察肝组织病理学改变。结果20周末试验结束时,与正常对照组相比,模型组肝指数、血清ALT、AST、ALP、TP、TC、LDL水平显著升高(P≤0.01),肝组织脂肪变性、炎症及纤维化程度均较对照组重(P≤0.01)。醋柳黄酮治疗组血清ALT、AST、TC较模型对照组明显降低(P<0.05),ALP、GGT、TG等也有所下降,但差异无统计学意义;肝纤维化程度较模型组明显减轻(P≤0.01),而肝组织脂肪变及炎症程度较模型组有改善趋势,但差异无统计学意义。醋柳黄酮治疗组大鼠血清脂质过氧化产物MDA含量较模型组明显下降,差异有统计学意义(P<0.05),血清SOD活性较模型组有升高趋势,但差异无统计学意义。绪论醋柳黄酮对高脂饮食大鼠非酒精性脂肪性肝病有一定的治疗作用。
Objective To study the effect of tilapia flavonoids on non-alcoholic fatty liver disease in rats. Methods Totally 54 male Sprague-Dawley rats were randomly divided into two groups. Normal control group was fed with normal diet, while the rest rats were fed with high fat diet (88% normal diet + 10% lard + 2% cholesterol). Normal control group and high-fat diet rats, 10 rats were sacrificed at the end of 12 weeks to observe the modeling effect. Twenty-four remaining high-fat diet rats were divided into model control group and tilapia flavonoids treatment group. The rats in the treatment group were given gavage with litchi (10mg / d) for 13 weeks, and the rats in each group were sacrificed at the end of the 20th week. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) GGT, ALP, TP, TG, TC, LDL, high-density lipoprotein (HDL), malondialdehyde (MDA) and superoxide dismutase (SOD) activity were observed. Liver histopathology was also observed. Results At the end of the 20-week trial, liver index, serum ALT, AST, ALP, TP, TC and LDL levels in model group were significantly higher than those in normal control group (P≤0.01), hepatic steatosis, inflammation and fibrosis The degree of weight than the control group (P ≤ 0.01). The levels of ALT, AST and TC in the serums of the three treatments were significantly lower than those of the model control group (P <0.05), and the levels of ALP, GGT and TG were also decreased, but the differences were not statistically significant; the degree of liver fibrosis was significantly reduced compared with the model group P≤0.01), while the degree of hepatic steatosis and inflammation were improved than the model group, but the difference was not statistically significant. Compared with the model group, MDA content of the serum lipid peroxidation product in the tilitasin-treated group was significantly decreased (P <0.05), and the serum SOD activity increased compared with the model group, but the difference was not statistically significant. INTRODUCTION Tilapia flavonoids have a certain therapeutic effect on nonalcoholic fatty liver disease in high-fat diet rats.