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建立了超高效液相色谱-串联质谱(UPLC-MS/MS)测定氟唑菌苯胺在小麦植株中内吸传导特性的方法;定量分析了分别在小麦根部和叶部两种施药方式下氟唑菌苯胺进入植株后的分布和累积情况。植株样品前处理采用Qu ECh ERS法,在反相色谱柱C18(100 mm×2.1 mm,1.8μm)上,以0.1%甲酸水和乙腈为流动相,利用梯度洗脱,正离子电离(ESI+),采用多反应离子监测模式(MRM)进行定性和定量。用基质匹配标准溶液外标法定量。结果表明:在0.5~100μg/L内,氟唑菌苯胺的质量浓度与对应的峰面积间线性关系良好,线性回归方程为y=12808.4x+308.518(r=0.9998)。在0.005、0.05和0.1mg/kg添加水平下,氟唑菌苯胺在小麦植株中的回收率为92%~126%,相对标准偏差为0.5%~9.1%;以3倍信噪比计算,方法检出限为0.037μg/kg;最低添加浓度为0.005 mg/kg。研究结果表明,氟唑菌苯胺于小麦植株根部施用后,可迅速在根部吸收并向茎部传导,且随处理浓度增大,药剂在不同部位的累积量增加;随处理后时间延长,药剂在不同部位的累积表现为快速吸收而后缓慢平衡。其中以0.01 mg/L氟唑菌苯胺经根部处理2 h后,药剂在根部、茎部和叶部含量分别为10.4、0.6和0.3μg/kg;氟唑菌苯胺经叶部单叶片施药后,亦可快速被叶片吸收,并可以跨叶传导至其他叶片,进而在茎部和根部累积。随处理后时间延长,施药叶片药剂快速下降并达到平衡,其他植株部位药剂快速累积并达到平衡。其中以200 mg/L的氟唑菌苯胺经第2片叶施用20μL,2 h后氟唑菌苯胺在根部和茎部的含量分别为109.0和148.0μg/kg;在第3片叶和旗叶中的含量分别为904.0和112.6μg/kg。
A method for the determination of fludazole aniline transport in wheat plants by UPLC-MS / MS was established. Distribution and accumulation of pyradenilide into plants. The plant samples were pretreated by Qu ECh ERS method using gradient elution and positive ionization (ESI +) on a reversed phase column C18 (100 mm × 2.1 mm, 1.8 μm) using 0.1% formic acid in water and acetonitrile as the mobile phase. , Using multi-reactive ion monitoring mode (MRM) for qualitative and quantitative. Matrix matching standard solution of external standard method. The results showed that there was a good linear relationship between the concentration of triflum aniline and the corresponding peak area in the range of 0.5-100 μg / L. The linear regression equation was y = 12808.4x + 308.518 (r = 0.9998). At the levels of 0.005, 0.05 and 0.1 mg / kg, the recoveries of phenformin in wheat plants ranged from 92% to 126% and the relative standard deviations were from 0.5% to 9.1%. The three-fold signal to noise ratio The detection limit was 0.037μg / kg; the lowest concentration was 0.005 mg / kg. The results showed that trimethoprim could be quickly absorbed in the roots and transferred to the stems after the roots of wheat plants were treated with fluconazole aniline. With the increase of treatment concentrations, the accumulation of pesticides in different parts increased. With the prolongation of treatment time, The cumulative performance of different parts of rapid absorption and then slowly balanced. Among them, the content of pesticide in roots, stems and leaves were 10.4, 0.6 and 0.3 μg / kg respectively after treatment with 0.01 mg / L penflufen for 2 h; , Can also be quickly absorbed by the leaves, and can be conducted across leaves to other leaves, thus accumulating in the stems and roots. With the prolongation of treatment time, the chemical components of the leaves rapidly decreased and reached a balance, and other plant parts rapidly accumulated and reached equilibrium. Among them, 20μL of the second trimetazidine was treated with 200mg / L triflumilamide, and the content of triflumilazole in the roots and stems was 109.0 and 148.0μg / kg after 2 hours respectively. In the third leaf and the flag leaf In the content of 904.0 and 112.6μg / kg respectively.