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作者采用单克隆抗体(McAB)标记等方法发现人类T淋巴细胞的新亚类,它们约占正常外周血T细胞的2%,称之为OKT3~+4~-8~-细胞。作者首先用密度梯度离心法分离出比重最大的细胞(d>1.069g/cm~3),约占总T细胞20%。然后再用各种McAB进行标记,发现一类能与总T细胞McAB相结合[如OKT3、Hu Lyt 3[9.6)和Leu 1],能形成E花结,但不能与T细胞亚类Mc AB(如OKT 4,OKT8,Leu 2A和Leu 3A)相结合的新亚类。在荧光激活分类器上进行分离的这些细胞比重大,偏向阴极端,不结合OKT4或OKT8抗体,能结合OKT3抗体,
The authors identified a new subclass of human T lymphocytes using monoclonal antibodies (McAB), which account for about 2% of normal peripheral blood T cells and are called OKT3 ~ + 4 ~ -8 ~ - cells. The authors first isolated the cells with the highest specific gravity (d> 1.069 g / cm ~ 3) by density gradient centrifugation, accounting for about 20% of total T cells. Then labeled with various McABs and found that a class of E-knot that binds to total T-cell McAB [eg, OKT3, Hu Lyt3 [9.6], and Leu1] can not associate with the T cell subset Mc AB (Such as OKT 4, OKT8, Leu 2A and Leu 3A) combination of new subcategories. These cells, which are isolated on a fluorescence-activated classifier, are more specific, prefer to the cathode, do not bind OKT4 or OKT8 antibodies, bind OKT3 antibodies,