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利用卡介苗（BCG）65KD热休克蛋白作为抗原，ELISA法检测Ⅰ型糖尿病患者血清中64KD抗体。对30例Ⅰ型糖尿病、30例Ⅱ型糖尿病、5例系统性红斑狼疮（SLE）、8例甲状腺机能亢进（甲亢）的患者及44例健康人血清进行检测。结果表明，64KD抗体在Ⅰ型糖尿病中的检出率为83．3％，在Ⅱ型糖尿病中的检出率为13．3％，在SLE及甲亢中均为阴性，健康人仅1例阳性。此方法的批内变异系数为4．57％～8．62％，批间变异系数为5．57％～12．7％。诊断特异性为94．6％，诊断敏感性为83．3％，诊断指数为177．9％。
The BCG 65KD heat shock protein was used as antigen, and the serum anti-64KD antibody was detected by ELISA in patients with type I diabetes. Serum samples were collected from 30 patients with type 1 diabetes mellitus, 30 patients with type 2 diabetes mellitus, 5 patients with systemic lupus erythematosus (SLE) and 8 patients with hyperthyroidism (Hyperthyroidism) and 44 healthy controls. The results showed that the detection rate of 64KD antibody was 83.3% in type I diabetes mellitus and 13.3% in type II diabetes mellitus, negative in both SLE and hyperthyroidism, and only 1 patient was positive in healthy people . The variation coefficient of this method was 4.57% ~ 8.62%, and the variation coefficient between lots was 5.57% ~ 12.7%. The diagnostic specificity was 94.6%, the diagnostic sensitivity was 83.3%, and the diagnostic index was 177.9%.