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目的探讨膳食纤维(DF)对锰毒作用的影响。方法48只3周龄雄性SD大鼠随机分为对照组、单纯DF饲食组、单纯染锰组(50mg/kg)、染锰+16%、8%和4%的DF饲食组(即分别在饲料中参入16%、8%和4%的膳食纤维)。1个月后神经行为测试出现明显改变后断头处死大鼠。测定脑、肝组织和血液(血清)中锰的含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与单纯染锰组比较,对照组、染锰+16%、8%DF组Morris水迷宫逃逸潜伏期缩短(P<0.01);跳台实验下台潜伏期延长(P<0.01),错误次数减少(P<0.01);脑、肝组织和血液(血清)中锰含量下降、SOD活性升高、MDA含量下降(P<0.01)。结论在本试验条件下,消化道染锰能引起大鼠神经行为异常和氧化损伤,在饲料中加入膳食纤维能抑制锰吸收及减少锰蓄积,对锰引起的氧化损伤和神经行为异常有抑制作用。
Objective To investigate the effect of dietary fiber (DF) on manganese toxicity. Methods Forty-eight male Sprague-Dawley rats of 3 weeks age were randomly divided into control group, DF fed group, DF group fed manganese alone (50mg / kg), DF fed 16%, 8% and 4% Respectively, in the feed into the 16%, 8% and 4% dietary fiber). One month after the neurobehavioral test changed significantly, rats were decapitated. The content of manganese, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in brain, liver tissue and blood (serum) were measured. Results The latent period of Morris water maze escape in control group, 16% of manganese-treated group and 8% of DF group was shorter than that of control group (P <0.01). The latency of step-down test was longer (P <0.01) 0.01). The content of manganese, the activity of SOD and the content of MDA of brain, liver and serum decreased (P <0.01). Conclusion Under the test conditions, manganese in the digestive tract can cause neurobehavior and oxidative damage in rats. Adding dietary fiber to the diet can inhibit the absorption of manganese and reduce the accumulation of manganese, which can inhibit oxidative damage and neurobehavioral abnormalities caused by manganese .