论文部分内容阅读
目的探讨感染肠道病毒71型(EV71)死亡病例的EV71 VP1基因特征学特征,为公共卫生管理机构及时加强EV71病原学监测提供决策依据。方法收集南平市近年来8例因EV71感染死亡的患儿的标本,进行EV71荧光定量RT-PCR鉴定,采用RT-PCR对6株分离到的EV71进行VP1基因编码区扩增,并对扩增产物进行核苷酸序列测序和分析。根据VP1测序结果与国内外报道的各基因型和基因亚型EV71 VP1序列进行同源性和亲缘进化分析。结果南平市手足口病重症死亡病例的病原体为肠道病毒EV71型C4亚型,与2010年-2012年浙江、江苏及上海流行的为同一亚型,同源性较高。结论 EV71病毒是引起手足口病重症病例的主要病原,通过基因分析发现在153 nt、156 nt处发现了新的点突变类型,在这2个位点出现的碱基T在NCBI数据库中未曾发现,不同位点氨基酸的突变可能成为死亡病例形成的生物学基础。
Objective To investigate the characteristics of EV71 VP1 gene in the death of EV71 and to provide a basis for public health agencies to enhance the etiological surveillance of EV71 in a timely manner. Methods Eight samples of children died of EV71 infection in Nanping City in recent years were collected and identified by EV71 fluorescent quantitative RT-PCR. The 6 EV71 isolated strains were amplified by RT-PCR, The product was sequenced and analyzed for nucleotide sequence. According to VP1 sequencing results, homology and phylogenetic analysis were carried out with the EV71 VP1 sequences of genotypes and subtypes of EV71 reported at home and abroad. Results The pathogen of severe death from hand-foot-mouth disease in Nanping City was enterovirus EV71 type C4 subtype, which shared the same homology with that of Zhejiang province, Jiangsu province and Shanghai city from 2010 to 2012. Conclusion The EV71 virus is the major causative agent of HFMD. A new type of point mutation was found at 153 nt and 156 nt by gene analysis. The nucleotide T at these two sites was not found in the NCBI database Amino acid mutations at different sites may be the biological basis for the formation of death cases.