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目的：探讨脑益嗪抗运动病作用的神经中枢机理与脑细胞ｃ－ｆｏｓ基因表达的关系。方法：采用正负交变加速度旋转刺激ＳＤ大鼠制备运动病模型，为运动病组；在脑益嗪组，ＳＤ大鼠预先注射脑益嗪，１小时后如运动病组进行旋转刺激；正常ＳＤ大鼠作为对照组。用ｃ－ｆｏｓ基因探针对３组大鼠的大脑皮质、小脑皮质及脑干前庭区进行原位杂交。用免疫组化方法检测３组大鼠脑组织中Ｆｏｓ蛋白的含量。观察运动病组大鼠和脑益嗪组大鼠大脑皮质、小脑皮质和脑干前庭区脑细胞ｃ－ｆｏｓ基因表达变化。结果：大鼠实施刺激后这三种脑组织中ｃ－ｆｏｓ转录水平增高及Ｆｏｓ蛋白含量均增高。在脑益嗪组ｃ－ｆｏｓ基因表达水平和Ｆｏｓ蛋白含量均低于运动病组。结论：脑益嗪抗运动病的中枢机理之一可能与ｃ－ｆｏｓ基因快速表达有关。
OBJECTIVE: To investigate the relationship between the neurotransmitter mechanism of cerebral ischemia and the c-fos gene expression in brain cells. Methods: The SD rats were induced by alternating positive and negative acceleration rotation to prepare motion sickness model, which was a motion sickness group. In the brain-yinzine group, SD rats were pre-injected with brain-yinzine and stimulated by rotational stimulation in motion sickness group one hour later. Normal SD rats as control group. The c-fos gene probes were used to in situ hybridize the cerebral cortex, cerebellar cortex and brainstem vestibular area in 3 groups of rats. Immunohistochemistry was used to detect Fos protein content in brain tissue of the three groups. To observe the changes of c-fos gene expression in cerebral cortex, cerebellar cortex and brainstem vestibular area of rats in motion sickness group and cerebral ischemia group. Results: The c-fos transcription and Fos protein contents of the three brain tissues were increased after stimulation in rats. The cerebral c-fos gene expression and Fos protein content in cerebral cilostazol group were lower than that of motion sickness group. CONCLUSION: One of the central mechanisms of brain-derived anti-motion sickness may be related to the rapid expression of c-fos gene.