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应用乙酰胆碱酯酶染色,乙醛酸诱发荧光及免疫过氧化物酶(PAP)法,采用铺片和切片,对猫的胆道系统植物性神经分布进行了观察。证明猫的胆道系统植物性神经由胆碱能、肾上腺素能及肽能神经组成两个神经节丛,即粘膜下神经节丛和浆膜下神经节丛。肾上腺素能神经分布于胆囊的浆膜下和粘膜固有层,参与上皮下丛和血管周神经丛的构成。生化检测组织中去甲肾上腺素(NA)含量结果表明:胆囊管NA含量最高,与其它各部位相比差异非常显著(P<0.01),其余各部之间差异不显著(P>0.05)。猫的胆道系统各层亦存在舒血管肠肽(VIP)、P物质(SP)、亮啡肽(L-ENK)及生长抑素(SOM)四种多肽免疫反应性神经,其中VIP最丰富,SP和L-ENK较少,SOM最稀疏,四种肽均以胆胰管括约肌处最为致密。本文并结合肽能神经对胆道系统的作用进行了讨论。
Acetylcholinesterase staining, glyoxylate induced fluorescence and immunoperoxidase (PAP) assay were used to observe the distribution of biliary plant bodily nerves in cats. The biliary system autonomic nerve of a cat shows that two ganglion plexus, the submucosal ganglion plexus and the subserosal ganglion plexus, are composed of cholinergic, adrenergic, and peptidergic nerves. Adrenergic nerve distribution in the gallbladder submucosal and mucosal lamina propria, participate in the formation of epithelial and perivascular plexus. The results of noradrenaline (NA) content in biochemical test showed that the content of NA was the highest in cystic duct, and the difference was significant compared with other parts (P <0.01). There was no significant difference between other groups (P> 0.05). There are four kinds of immunoreactive nerves, vasoactive intestinal peptide (VIP), substance P (SP), leucomans (L-ENK) and somatostatin (SOM) SP and L-ENK less, SOM most sparse, the four peptides are the most compact at the bile duct sphincter. This article and the peptide nerve in the biliary system is discussed.