论文部分内容阅读
为筛选与小麦抗白粉病基因Pm2紧密连锁的分子标记,将感病品种Chancellor与Pm2的近等基因系杂交,获得F1、F2分离群体,采用分离群体分组法对Pm2进行了微卫星(m icrosatellite,又称simple sequence repeats,SSR)标记分析。结果表明,定位于小麦5D染色体上的71对SSR引物中有12对引物能在Pm2的近等基因系、Chancellor间稳定地揭示出多态性差异,7对引物Xcfd189、Xcfd29、Xcfd8、Xcfd102、Xcfd7、Xcfd57和Xgwm190分别能在抗病、感病池间和F2分离群体的抗病、感病单株间稳定地扩增出特异性产物。7对引物所扩增的特异谱带分别为:Xcfd189360、Xcfd29190、Xcfd8160、Xcfd102250、Xcfd7200、Xcfd57245和Xgwm190210,它们与Pm2基因间的遗传距离分别为0、1.5、2.3、5.4、10.2、31.5和54.3 cM,其中标记Xcfd189360与Pm2共分离,标记Xcfd 29190、Xcfd8160和Xcfd102250与Pm2紧密连锁,可用于Pm2的标记辅助选择。
In order to screen molecular markers closely linked to Pm2, the susceptible cultivar Chancellor was crossed with the near-isogenic lines of Pm2 to obtain F1 and F2 segregating populations. Pm2 was microsatellite , Also known as simple sequence repeats, SSR) marker analysis. The results showed that 12 of 71 pairs of SSR primers located on chromosome 5D of wheat could stably reveal the polymorphism among the Pm2 near-isogenic lines and Chancellor. Seven pairs of primers Xcfd189, Xcfd29, Xcfd8, Xcfd102, Xcfd7, Xcfd57 and Xgwm190 could stably amplify the specific products in the resistant and susceptible plants of resistant and susceptible pools and F2 segregating populations respectively. The specific bands amplified by 7 pairs of primers were: Xcfd189360, Xcfd29190, Xcfd8160, Xcfd102250, Xcfd7200, Xcfd57245 and Xgwm190210, respectively. The genetic distances between them and Pm2 were 0, 1.5, 2.3, 5.4, 10.2, 31.5 and 54.3, respectively cM, in which the marker Xcfd189360 was co-segregated with Pm2. The markers Xcfd 29190, Xcfd8160 and Xcfd102250 were closely linked with Pm2 and could be used for marker-assisted selection of Pm2.