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目的探讨巨细胞病毒诱导的β-分泌酶在小鼠体内的变化及在藻酸双酯钠(PSS)治疗后淀粉样蛋白前体(APP)和β-分泌酶(BACE-1)的表达。方法将30雄性只小鼠随机分为3组(每组10只)。对照组小鼠不做任何处理;CMV感染组,腹腔一次性注射MCMV 0.2mL,30d后处死;PSS治疗组,CMV感染后,每日灌胃PSS 60mg/kg,持续14至30d。在第30天,处死所有的小鼠。通过蛋白质免疫印迹和免疫组化检测β-分泌酶的表达。结果 APP和BACE-1在感染小鼠脑中的表达显著高于PSS处理后,与感染组相比蛋白表达降低。结论 PSS可通过降低小鼠巨细胞病毒诱导的β-分泌酶的表达水平,缓解继发性脑损伤程度,并在未来的治疗中起到保护神经作用。
Objective To investigate the changes of cytomegalovirus-induced β-secretase in mice and the expression of amyloid precursor protein (APP) and β-secretase (BACE-1) after treatment with sodium alginate (PSS). Methods Thirty male mice were randomly divided into 3 groups (10 in each group). The mice in the control group were treated with no CMV infection. MCMV 0.2 mL was intraperitoneally injected into the abdominal cavity for 30 days and then sacrificed. PSS-treated mice were given PSS 60 mg / kg daily for 14 to 30 days after CMV infection. On the 30th day, all mice were sacrificed. Beta-secretase expression was detected by Western blotting and immunohistochemistry. Results The expression of APP and BACE-1 in the brain of infected mice was significantly higher than that of PSS, and the protein expression was decreased compared with the infected group. Conclusion PSS can relieve the degree of secondary brain injury by decreasing the expression level of β-secretase induced by mouse cytomegalovirus and play a neuroprotective role in the future treatment.