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为探讨一氧化氮(Nitric oxide,NO)信号在脱落酸(Abscisic acid,ABA)诱导丹参酚酸类成分积累中的作用,采用不同浓度一氧化氮外源供体硝普钠(Sodium nitroprusside,SNP)处理丹参毛状根,6 d后采收,测定酚酸类成分含量;ABA联合一氧化氮清除剂(2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide,c-PTIO)或一氧化氮合酶抑制剂(N~G-nitro-L-arginine methyl ester,L-NAME)对丹参毛状根进行处理,测定酚酸类成分含量和关键基因表达量。结果表明,100μmol/L SNP对丹参毛状根中迷迭香酸与丹酚酸B积累的诱导效果最显著,迷迭香酸和丹酚酸B含量分别增加了3倍和4倍。ABA处理能显著促进PAL(Phenylalanine ammonia lyase)、TAT(Tyrosine aminotransferase)和RAS(Rosmarinic acid synthase)基因的表达,促进丹参毛状根中酚酸类成分的积累,而联合c-PTIO或L-NAME共同处理后,3种关键基因表达下调,并显著抑制了酚酸类成分的积累。研究证明NO和ABA均能够促进丹酚酸类成分的积累,NO信号可能介导了ABA对丹酚酸生物合成的诱导作用。
To investigate the role of nitric oxide (NO) signaling in the accumulation of salvianolic acids in Abscisic acid (ABA) -induced pancreatic cancer cells, different concentrations of nitric oxide donor, sodium nitroprusside (SNP ) Treatment of hairy roots of Salvia, 6 d after harvest, determination of phenolic acids content; ABA combined with nitrogen monoxide scavenger (2- (4-carboxy-2-phenyl) -4,4,5,5-tetramethylimidazoline- 1-oxyl-3-oxide (c-PTIO) or nitric oxide synthase inhibitor (N-G-nitro-L-arginine methyl ester, L-NAME) Content and key gene expression levels. The results showed that 100μmol / L SNP had the most significant effect on the accumulation of rosmarinic acid and salvianolic acid B in Salvia miltiorrhiza hairy roots, while the content of rosmarinic acid and salvianolic acid B increased 3-fold and 4-fold respectively. ABA treatment could significantly promote the expression of Phenylalanine ammonia lyase (PAL), TAT (Tyrosine aminotransferase) and RAS (Rosmarinic acid synthase) genes, and promote the accumulation of phenolic acids in hairy roots of Salvia miltiorrhiza, but not with c-PTIO or L-NAME After co-treatment, the expression of three key genes was down-regulated and the accumulation of phenolic acids was significantly inhibited. Studies have shown that NO and ABA can promote the accumulation of salvianolic acids, NO signal may be mediated ABA induction of salvianolic acid biosynthesis.