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植原体是一类寄生于植物韧皮部的原核生物。目前国际上主要根据植原体16S rDNA RFLP图谱的比对进行分类及鉴定。传统的PCR-RFLP试验步骤繁琐、重复性差,误差大,尤其在检测鉴定大量植原体病原时,耗时、耗力。通过对目前已公布的全部植原体16S rDNA序列进行比对整理,并生成16S rDNA RFLP电子指纹图谱库。在此基础上开发了指纹图谱分析软件DNA-FP Cluster1.0。该软件可基于序列或图谱形式进行比对,并自动输出比对结果。结果呈现形式为3种:即一种16S rDNA RFLP图谱与多种图谱间相似性分析;多种16S rDNA RFLP图谱间相似性分析;16S rDNA RFLP图谱间相似度树状图。该软件在不需要酶切试验操作的情况下实现了对植原体的快速分类与鉴定,并解决了因植原体材料不全而无法进行植原体酶切后比对的难题,为今后植原体候选种内的细化分类提供了方法与工具。
Phytoplasma is a type of prokaryotic parasites in the phloem of plants. At present, the classification and identification of phytoplasma 16S rDNA RFLP maps are mainly classified and identified in the world. Traditional PCR-RFLP test steps cumbersome, poor reproducibility, error, especially in the detection of pathogenicity of a large number of phytoplankton, time-consuming and labor-consuming. By comparing 16S rDNA sequences of all the phytoplasmas that have been published to date, a 16S rDNA RFLP electronic fingerprint database was generated. On this basis, DNA fingerprinting analysis software DNA-FP Cluster1.0 has been developed. The software can be based on sequence or map form of comparison, and automatically output the results of the comparison. The results presented in three forms: a 16S rDNA RFLP map and a variety of atlas similarity analysis; a variety of 16S rDNA RFLP atlas similarity analysis; 16S rDNA RFLP atlas similarity tree. The software does not require enzyme digestion test operation to achieve rapid classification and identification of phytoplasma, and to solve the phytoplasma due to incomplete material and can not be phytoplasma digested after the comparison of the problem, for future plant species candidates Within the thin classification provides methods and tools.