目的:建立一个简便、灵敏度高、重现性好的测定鸡蛋中雌二醇、雌三醇、炔雌醇的方法。方法:新鲜鸡蛋打碎均匀后,用乙腈提取两次,然后目标化合物以水/甲醇/乙腈(50∶30∶20,v/v/v)为流动相,过XTerra RP18色谱柱(5μm,4.6 mm×150 mm),荧光检测器进行检测。结果:3种雌激素的检出限为0.04 mg/kg～0.12 mg/kg,鸡蛋样品中雌激素的添加浓度在0.8 mg/kg～16 mg/kg范围内,组分的平均回收率范围在80.6%～94.4%,相对标准偏差为1.52%～5.11%。结论:该方法简便、准确并可同时测定鸡蛋中的雌激素。 Objective: To establish a simple, sensitive and reproducible method for the determination of estradiol, estriol and ethinylestradiol in chicken eggs. Methods: The fresh eggs were homogenized and extracted twice with acetonitrile. The target compounds were eluted with XTerra RP18 (5μm, 4.6 mm × 150 mm), fluorescence detector for detection. Results: The detection limit of three estrogens was 0.04 mg / kg ~ 0.12 mg / kg, and the concentration of estrogen in egg samples was in the range of 0.8 mg / kg ~ 16 mg / kg. The average recoveries of the components ranged from 80.6% ~ 94.4%, with relative standard deviations of 1.52% ~ 5.11%. Conclusion: The method is simple, accurate and simultaneous determination of estrogen in eggs.