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目的:研究小檗碱对高脂血症大鼠的调脂作用及对肝脏细胞CPTⅠA基因表达的影响。方法:按血脂高低将大鼠随机分为正常组,高脂模型组,小檗碱3006,0 mg.kg-1.d-1组和洛伐他汀7.2 mg.kg-1.d-1组。正常组用基础饲料喂养,其余各组喂以高脂、高胆固醇饲料,制备实验性高脂血症模型。于给药12周,分别取血样,采用全自动生化分析仪测定血脂变化;以RT-PCR和Western blot法评价大鼠肝脏细胞CPTⅠA mRNA和蛋白表达以及PPARαmRNA表达。结果:与高脂模型组相比,小檗碱呈剂量依赖性明显阻遏总胆固醇(TC)及低密度脂蛋白胆固醇(LDL-C)水平的增加和提升高密度脂蛋白胆固醇(HDL-C)水平;明显促进CPTⅠA mRNA和蛋白表达,但对PPARαmRNA表达无明显影响;洛伐他汀也显著阻遏TC,TG,LDL-C水平增加和提升HDL-C水平,但对CPTⅠA和PPARα基因表达均无明显影响。结论:小檗碱具有良性调血脂作用,其作用机制可能涉及与促进脂质代谢相关的CPTⅠA基因表达有关。
OBJECTIVE: To study the effect of berberine on lipid regulation of lipopolysaccharide-induced hyperlipidemic rats and its effect on the expression of CPTⅠA gene in liver cells. Methods: The rats were randomly divided into normal group, high fat model group, berberine 3006,0 mg.kg-1.d-1 group and lovastatin 7.2 mg.kg-1.d-1 group . The normal group was fed with basal diet, while the other groups were fed with high fat and high cholesterol feed to prepare experimental hyperlipidemia model. At 12 weeks after administration, blood samples were taken and the changes of blood lipids were measured by automatic biochemical analyzer. The mRNA and protein expression of CPTⅠA and the mRNA expression of PPARα in rat liver cells were evaluated by RT-PCR and Western blot. Results: Berberine significantly inhibited the increase of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) and the increase of high density lipoprotein cholesterol (HDL-C) in a dose- , But significantly reduced the mRNA expression of CPTⅠA and PPARαmRNA. Lovastatin also significantly inhibited the increase of TC, TG, LDL-C and HDL-C levels, but had no significant effect on the expression of CPTⅠA and PPARα influences. Conclusion: Berberine has a good blood lipid regulating effect, and its mechanism may be related to the promotion of lipid metabolism related CPT Ⅰ A gene expression.