论文部分内容阅读
为了测定重组鼠干细胞因子(SCF)对小鼠原始造血干细胞的体内作用,作者将经150mg/kg体重的5-氟尿嘧啶处理5天后的CBA/J雄性小鼠骨髓细胞输注给经致死剂量照射同系雌性小鼠,移植后立即连续6天皮下单独或联合注射1.0μg/鼠/d的SCF、0.2μg/鼠/d的IL-3和0.2μg/鼠/d的GM-CSF,12天后测定受体小鼠的脾结节形成单位
In order to determine the in vivo effect of recombinant murine stem cell factor (SCF) on mouse hematopoietic stem cells in vivo, we administered bone marrow cells from CBA / J male mice treated with 5-fluorouracil at 150 mg / kg body weight for 5 days to the lethal dose irradiated Female mice were injected subcutaneously with 1.0 μg / mouse / d of SCF, 0.2 μg / mouse / d of IL-3 and 0.2 μg / mouse / d of GM-CSF subcutaneously for 6 days immediately after transplantation, Body mouse spleen nodules forming units