论文部分内容阅读
背景:莨菪碱类药物改善微循环和作为中药麻醉剂已在国内临床上广泛应用,但莨菪类生物碱抗吗啡成瘾作用的动物实验缺乏深入报道。目的:观察莨菪类生物碱与吗啡合用对抗吗啡致小鼠依赖性的作用,以期为开发防治阿片类物质成瘾的莨菪类药物提供实验学依据。设计:以实验动物为研究对象的随机对照实验。单位:一所大学医学院的生理学实验室。材料:本实验于2004-06/2004-08在河北工程学院医学部生理学实验室完成。选择健康雄性昆明小鼠50只,2月龄,体质量(20±2)g,由河北医科大学实验动物中心提供,清洁级。方法:依据吗啡成瘾动物的依赖性评价指标,选择痛阈和纳络酮跳台反应作为观察项目。将50只小鼠随机分为5组,即对照组、吗啡组、东莨菪碱组、山莨菪碱组和阿托品组,每组10只。分别于第1天至第7天每天用热板法观察腹腔注射生理盐水、吗啡及莨菪类生物碱合并吗啡后1h的痛阈。并于末次(第7天)给药后6h,腹腔注射纳络酮5mg/kg催促,观察30min内小鼠跳跃次数。主要观察指标:①吗啡成瘾小鼠的痛阈;②吗啡成瘾小鼠的跳跃次数和跳跃动物数(率)。结果:与对照组比较,吗啡组小鼠的痛阈明显下降(P<0.05或P<0.01),跳跃次数和跳跃动物率明显增加。东莨菪碱与吗啡合用7d后,明显提高吗啡依赖小鼠的痛阈(P<0.01),并减少跳跃次数和跳
BACKGROUND: Scopolamine has been widely used clinically in China to improve microcirculation and as an anesthetic in traditional Chinese medicine. However, animal experiments on antineoplastic effects of scopolamine alkaloids have not been reported in depth. OBJECTIVE: To observe the dependence of morphine alkaloids and morphine on morphine-induced mice and to provide experimental basis for the development of antiepidensis drugs for preventing and treating opioid addiction. Design: A randomized controlled study of experimental animals. Unit: a University College of Physiology Laboratory. Materials: The experiment was performed at the Physiology Laboratory of Medical College of Hebei Institute of Engineering from June 2004 to August 2004. Fifty healthy male Kunming mice were selected, 2 months old, body weight (20 ± 2) g, provided by Experimental Animal Center of Hebei Medical University, clean grade. Methods: Based on the dependence of morphine addicted animals, we selected the pain threshold and naloxone jumping reaction as observation items. Fifty mice were randomly divided into five groups: control group, morphine group, scopolamine group, anisodamine group and atropine group, with 10 mice in each group. The pain threshold at 1 hour after intraperitoneal injection of morphine, morphine and alkaloid combined with morphine was observed daily by hot plate method on day 1 to day 7 respectively. At 6h after the last administration (day 7), intraperitoneal injection of naloxone (5mg / kg) was performed to observe the number of mice jumping within 30min. MAIN OUTCOME MEASURES: ① Pain threshold in morphine-addicted mice; ② Jumping number and number of jumping animals in morphine-addicted mice. Results: Compared with the control group, the pain threshold in morphine group was significantly decreased (P <0.05 or P <0.01), and the number of jumping and jumping animals increased significantly. Scopolamine combined with morphine significantly increased the pain threshold of morphine-dependent mice (P <0.01) and reduced the number of jumps and jumps