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在前一报告已简述我们用乙醇分段抽提法从猪胸腺中制备粗提物,观察其体内免疫抑制效应。本文将粗提物通过Ultrogel ACA 54凝胶过滤提纯,获得部分纯化的制品。在PHA和ConA诱导下,对该制品的抑制效应进行了研究并对某些生化特性作了鉴定。通过ACA～54胶滤后分得3个峰,活力分布在Ⅱ～Ⅲ峰,将Ⅱ峰再层析又分得A和B两峰。活性较高的A峰聚丙烯酰胺凝胶电泳PAGE图谱显示一主要区带和另一小的区带。SDS-PAGE法测定其分子量分别为70,000和15,000道尔顿,用Alcian-Blue染色法证明这两条带均是糖蛋白。
In the previous report, we briefly described the preparation of crude extracts from porcine thymus by ethanol fractionation and observed the immunosuppressive effect in vivo. The crude extracts were purified by gel filtration on Ultrogel ACA 54 to obtain partially purified preparations. Under the induction of PHA and ConA, the inhibitory effect of the product was studied and some biochemical properties were identified. After ACA ~ 54 gel filtration, three peaks were obtained, and the vigor distribution was in Ⅱ ~ Ⅲ peak. The second peak was further separated into A and B peaks. The more active A-peak polyacrylamide gel electrophoresis PAGE pattern showed one major zone and another smaller zone. SDS-PAGE method to determine the molecular weight of 70,000 and 15,000 Daltons, Alcian-Blue staining showed that these two bands are glycoproteins.