为研究环卵沉淀试验虫卵抗原标准化,本文对不同感染期兔肝分离虫卵和感染血清作了定期的观察。 材料与方法 家兔20只,每兔接种尾蚴400～600条,于接种后35d后开始解剖,每隔5d解剖1只,取肝脏,经组织捣碎机捣碎,加0.25％胰蛋白酶,置电磁搅拌器,加热(55℃～60℃)搅拌消化后,离心沉淀分离虫卵,每批分离卵分别以三挡不同功率超声处理后制作热处理超声干卵抗原与重感染混合兔血清作 In order to study the standardization of egg antigen in ring-and-ovum precipitation test, we regularly observed the eggs and serums of rabbits in different stages of infection. MATERIALS AND METHODS Twenty rabbits were inoculated with 400-600 cercariae per rabbit, dissected 35 days after inoculation, and dissected 1d at 5d intervals. The liver was harvested and triturated with a tissue crushing machine. 0.25% trypsin was added Electromagnetic stirrer, heating (55 ° C ~ 60 ° C) after stirring and digestion, centrifugation and separation of eggs, each batch of eggs were sifted at different powers after three heat treatment ultrasound heat-dried egg antigen and heavy infection mixed rabbit serum