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采用聚合酶链反应(PCR)及激光扫描技术,对1期4例、Ⅱ期12例、Ⅲ期18例、Ⅳ期4例的卵巢癌组织及6例正常卵巢组织的 DNA 作 C-myc 基因扩增检测,分析不同临床分期、病理分化程度及淋巴结转移情况与 C-myc 基因扩增率之间相关性。结果,38例卵巢癌 C-myc 扩增率47%,癌旁组织40%,淋巴结转移的10例全部扩增,正常组织未有扩增.提示:1.C-myc 扩增与临床分期、病理分化程度、淋巴结转移密切相关。2.PCR-激光扫描技术与其它各种分子杂交技术相比,具有简单、快速、特异、没有放射性污染等优点,适合于临床应用。
Polymerase chain reaction (PCR) and laser scanning were used to detect the C-myc gene in 4 cases of stage I, 12 cases of stage II, 18 cases of stage III, 4 cases of stage IV ovarian cancer and 6 cases of normal ovarian tissue Amplification test, analysis of different clinical stages, pathological differentiation and lymph node metastasis and C-myc gene amplification rate between the correlation. Results 38 cases of C-myc ovarian cancer amplification rate of 47%, paracancer tissue 40%, lymph node metastasis of 10 cases of all amplification, normal tissue did not amplify.Caution: 1. C-myc amplification and clinical stage, Pathological differentiation, lymph node metastasis are closely related. Compared with other kinds of molecular hybridization techniques, PCR-laser scanning has the advantages of simple, rapid, specific, no radioactive contamination and is suitable for clinical application.