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在弱酸条件下,用二次石英蒸馏水为溶剂,核固红和红霉素在常温下可以反应形成稳定的离子缔合物,导致吸收光谱改变,且吸光度(以试剂空白作参比)与红霉素的浓度成正比。于535nm测定其吸光度得到ε=2.33×10~4L/mol·cm,红霉素的浓度在0.0009—0.050mg/mL范围内服从Beer定律,基于此,我们建立了一种测定红霉素的新方法。方法的检出限为0.10μg/mL。实验表明:该法简便可靠,重现性和选择性好,可用于红霉素肠溶片和红霉素片中红霉素含量的测定。
In weak acid conditions, the second quartz distilled water as the solvent, nuclear red and erythromycin at room temperature can react to form a stable ion association, resulting in changes in absorption spectra, and absorbance (reagent blank as a reference) and red The concentration ofmycin is proportional. The absorbance measured at 535 nm gave ε = 2.33 × 10 -4 L / mol · cm, and the concentration of erythromycin in the range of 0.0009-0.050 mg / mL obeys Beer’s law. Based on this, we established a new method for the determination of erythromycin method. The detection limit of the method is 0.10μg / mL. Experiments show that the method is simple and reliable, reproducible and selective, and can be used for the determination of erythromycin in erythromycin enteric-coated tablets and erythromycin tablets.