以麻醉狗进行实验,每次用实验狗和给血狗各1只。血从给血狗颈动脉输入实验狗心的左前降支和旋支,冠状窦血经右颈静脉流入左颈静脉,左股动脉血回流给血狗右股静脉。给实验狗右股静脉注射谷氨酸(3 mg/kg/min),用夹使其冠脉流量(CBF)约降低60%。进行对照的正常、缺血和再灌注实验,然后在谷 To anesthetize dogs experiment, each with experimental dogs and blood dogs each one. Blood was injected into the left anterior descending branch and the spinous branch of the experimental dog heart from the feeding dog carotid artery. The coronary sinus blood flowed into the left jugular vein via the right jugular vein and the left femoral artery returned to the right femoral vein of the blood dog. Glutamic acid (3 mg / kg / min) was injected into the right femoral vein of experimental dogs and their coronary flow (CBF) was reduced by approximately 60% using a clamp. Control normal, ischemic and reperfusion experiments were performed and then in the valley