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目的探讨泌尿生殖道沙眼衣原体(Ct)不同omp1基因型感染的临床特征。方法套式聚合酶链反应(Nested PCR)检测尿道(宫颈)拭子,诊断Ct omp1基因型感染,常规培养法检测泌尿道拭子中的淋球菌、解脲支原体/人型支原体,ELISA法检测单纯疱疹病毒,排除其他性传播感染。按照无症状感染、泌尿生殖道激惹症及异常分泌物三类临床表现,分析Ct各生物群、不同omp1基因型Ct感染的临床特征。结果共检测126例Ct阳性者,检出Ct 126株。宿主性别分析表明:三组生物群Ct中,各生物群Ct感染宿主时无性别选择性(P>0.05);所有感染者中,50%由B组感染,27.8%由C组感染,22.1%由中间组感染引起。对其中80例单纯Ct感染者的症状分析表明:无症状感染者中,女性多于男性(P<0.05);男性泌尿道感染omp1基因型D、F、J型常有症状(P=0.042<0.05);按生物组分析表明:B组、C组及中间组分别有50%、65.6%、55%感染者有症状,组间差异有统计学意义(χ2=8.67,P<0.05)。结论不同的Ct omp1基因型感染泌尿生殖道后,男女患者的症状和体征间有一定差异。
Objective To investigate the clinical features of different omp1 genotypes in genitourinary tract Chlamydia trachomatis (Ct). Methods Nested PCR was used to detect urethral (cervical) swabs to diagnose Ct omp1 genotype infection. Neisseria gonorrhoeae and Ureaplasma urealyticum were detected by routine culture method and ELISA Herpes simplex virus, excluding other sexually transmitted infections. According to the three clinical manifestations of asymptomatic infection, genitourinary irritability and abnormal secretion, we analyzed the clinical features of Ct infection in different Ct biota and different omp1 genotypes. Results A total of 126 Ct positive cases were detected and 126 Ct strains were detected. Host sex analysis showed that there was no sex-selective (P> 0.05) Ct in all three groups, and 50% of all infected were infected by group B, 27.8% were infected by group C, 22.1% Caused by the infection in the middle group. The symptom analysis of 80 patients with simple Ct infection showed that more women than men were asymptomatic (P <0.05), and common symptoms of omp1 genotypes D, F and J in male patients with urinary tract infection (P = 0.042 < 0.05). According to the bio-group analysis, the symptoms in 50%, 65.6% and 55% of patients in group B, C and intermediate group were statistically significant (χ2 = 8.67, P <0.05). Conclusion Different genotype Ct omp1 infection genitourinary tract, male and female patients have some differences between the symptoms and signs.