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目的 为了探讨低剂量辐射兴奋效应的分子机理 ,本文作者对昆明小鼠接受 75mGyX射线全身照射后 4h的脾细胞进行了蛋白分子表达的研究。方法 用凝胶过滤和高效液相色谱法 (HPLC)分析蛋白分子表达 ,通过3H TdR掺入的淋巴细胞转化和人外周血淋巴细胞染色体畸变率观察蛋白分子表达的生物活性。结果 发现相对分子质量为 170 0 0、185 0 0和 5 0 0 0 0 3种蛋白质的表达在照射后发生改变 ,这 3种蛋白质分别在脾细胞浆、全脾细胞和脾细胞核中发现 ,其中 170 0 0和 185 0 0的蛋白分子表达下调 ,5 0 0 0 0的蛋白分子表达上调。并且照射后 170 0 0和 185 0 0蛋白质分子表达下调的组分对正常脾细胞的增殖反应具有促进作用 ,185 0 0蛋白质分子表达下调的组分和 5 0 0 0 0蛋白分子表达上调的组分对人外周血淋巴细胞染色体辐射损伤具有保护作用。结论 低剂量辐射诱导蛋白分子表达的变化在低剂量辐射兴奋效应发生机理中可能具有重要作用
Objective To investigate the molecular mechanism of low-dose radiation excitatory effects, we investigated the expression of protein molecules in splenocytes of Kunming mice exposed to 75mGy X-ray 4h after irradiation. Methods Protein expression was analyzed by gel filtration and high performance liquid chromatography (HPLC). The biological activity of protein expression was observed by 3H TdR incorporation into lymphocytes and chromosome aberration rate of human peripheral blood lymphocytes. The results showed that the proteins with relative molecular masses of 170 0 0, 185 0 0 and 500 000 changed after irradiation, and these three proteins were found in the spleen cytoplasm, splenocyte and splenocyte nucleus, respectively 170 0 0 and 185 0 0 protein molecules were down-regulated, 50000 protein molecules were up-regulated. And the down-regulated components of 170 0 0 and 185 0 0 protein molecules promoted the proliferation of normal spleen cells after irradiation, and the down-regulated components of 185 0 0 protein molecules and the up-regulated 5 00 0 0 proteins Points on human peripheral blood lymphocyte chromosome damage has a protective effect. Conclusion The changes of low molecular weight radiation-induced protein molecular expression may play an important role in the mechanism of low-dose radiation excitotoxicity