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目的探讨亚砷酸钠对雄性生殖细胞生殖毒性的影响。方法选用雄性小鼠染砷,分批与正常雌性小鼠交配,观察雄性小鼠生精细胞发育不同阶段砷毒对受孕率、胚胎死亡率及体细胞嗜多染红细胞致突性的影响效应。结果染砷后第2、4周30mg/kg剂量组受孕率低于70%;染砷后第6周10、15、30mg/kg组胚胎死亡率分别为30.0%、38.9%、40.0%,仍明显高于阴性对照组16.7%;染砷剂量>10mg/kg时,周围血体细胞嗜多染红细胞致突性损伤恢复时间早于生殖细胞致突性损伤恢复时间。结论亚砷酸钠可作用于观察期内生精细胞发育的各个环节,以对精细胞发育作用最为明显,主要表现为受孕率随染毒剂量增加而降低,胚胎死亡率随剂量的增加而升高。
Objective To investigate the effects of sodium arsenite on reproductive toxicity of male germ cells. Methods The male mice were stained with arsenic and mated with normal female mice in batches to observe the effect of arsenite in different stages of spermatogenic cell development on the pregnancy rate, embryo mortality and somatic hyperchromatic erythrocytes induced by male mice. Results The first and second weeks after arsenic exposure 30mg / kg dose group, the pregnancy rate of less than 70%; the first 6 weeks after arsenic in 10,15,30 mg / kg group of embryonic mortality rates were 30.0%, 38.9%, 40.0% Significantly higher than the negative control group of 16.7%; arsenic dose of> 10mg / kg, peripheral blood cell polychromatic erythrocytes induced damage recovery time earlier than germ cell damage recovery time. Conclusion Sodium arsenite can act on all aspects of spermatogenic cell development during the observation period, and has the most obvious effect on the spermatogenesis. The main manifestations are that the pregnancy rate decreases with the increase of the dose, and the embryo mortality rate rises with the increase of dosage high.