论文部分内容阅读
目的采用HPLC测定清肝化淤合剂中槲皮素的含量。方法采用HypersilODS2色谱柱(250mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸水溶液(51:49),流速1.0mL·min-1,检测波长360nm,柱温25℃。结果槲皮素2.9～23.22μg·mL-1与峰面积呈良好的线性关系(r=0.9999),平均加样回收率为99.12%,RSD=1.81%。结论所建方法简便、快捷、准确度高,可用于清肝化淤合剂中槲皮素的测定。
Objective To determine the content of quercetin in Qinggan Huajin Mixture by HPLC. Methods The Hypersil ODS column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-0.4% phosphoric acid solution (51:49). The flow rate was 1.0 mL · min-1. The detection wavelength was 360 nm and the column temperature was 25 ℃. Results The peak area of 2.9 ~ 23.22μg · mL-1 quercetin showed a good linear relationship (r = 0.9999). The average recovery was 99.12% and RSD was 1.81%. Conclusion The method is simple, rapid and accurate. It can be used for the determination of quercetin in