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[Objective] To establish a method for HPLC fingerprint of dry roots of Angelica polymorpha Maxim. [Method] Chromatographic column was Kromasil 100A C18 (250 mm × 4.6 mm,5 μm); column temperature was 25 ℃; acetonitrile-water was used for gradient elution of mobile phase; flow rate was 1 mL/min; detection wavelength was 254 nm; injection volume was 10 μL; isoimperatorin was taken as the reference substance; and HPLC fingerprints of 10 batches of A. polymorpha were analyzed. [Result] The common pattern of HPLC fingerprints of A. polymorpha was established; there were in all 8 common peaks; and the similarity degrees of the 10 batches of A. polymorpha were all greater than 0.9. [Conclusion] This method was stable, accurate and simple, and provided scientific basis for the quality control and evaluation of A. polymorpha.