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目的:建立可靠、稳定的手针针刺镇痛效应平台。方法:选择佐剂性关节炎疼痛模型为研究平台,先后以2种不同浓度的造模药物以及3种手针针刺刺激参数开展了研究。结果:采用低浓度佐剂(含结核杆菌浓度为1 mg/m L)造模后24 h大鼠致炎侧足底痛阈明显下降,并且可持续到观察周期结束(造模后21 d);高浓度佐剂(10 mg/m L)造模后24 h大鼠致炎侧足底痛阈亦明显下降,但在造模后容易出现足底溃疡等不良反应,影响指标的监测。针刺单侧足三里穴,未见明显的镇痛效应;针刺双侧足三里穴,留针30 min(其间每隔5 min行捻转手法,平补平泻,180次/min,持续2min)可有效抑制佐剂性关节炎大鼠的疼痛症状。结论:采用低浓度佐剂造模,手针针刺双侧足三里穴可有效建立针刺治疗佐剂性关节炎疼痛模型效应平台。在此效应平台的建立过程中,存在多种影响因素,主要包括实验动物、造模药物浓度、针刺干预参数、效应指标等因素,在具体研究过程中,应当综合考虑这些因素。
Objective: To establish a reliable and stable hand acupuncture analgesic effect platform. Methods: The pain model of adjuvant arthritis was selected as the research platform. Two kinds of model drugs with different concentrations and three kinds of acupuncture stimulating parameters of hand acupuncture were studied. Results: The pain threshold of inflammatory side of the rats on 24 h after low-dose adjuvant (including Mycobacterium tuberculosis at a concentration of 1 mg / m L) was significantly decreased and continued to the end of the observation period (21 d after modeling) The hyperalgesia pain threshold decreased significantly at 24 h after injection of high concentration adjuvant (10 mg / m L), but adverse reactions such as plantar ulcer were easily observed after the model was established, which could affect the monitoring of the index. Acupuncture at one side of Zusanli acupoints, no obvious analgesic effect; acupuncture bilateral Zusanli acupoints, needle retention 30 min (during which every 5 min twisting hand, flat fill diarrhea, 180 beats / min, sustained 2min ) Can effectively inhibit the pain of adjuvant arthritis in rats. CONCLUSION: Anesthesia with adjuvant arthritis can be effectively established by using acupuncture at both sides of Zusanli acupoints with low concentration adjuvant. There are many influencing factors in the establishment of this effect platform, including experimental animals, drug concentration, acupuncture intervention parameters and effect indicators. These factors should be considered comprehensively in the process of specific research.