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目的:建立HPLC梯度法同时测定复方三七维康胶囊中三七皂苷R_1、人参皂苷R_(b1)、R_(g1)3种皂苷的含量。方法:色谱柱:大连Spherisorb C_(18)分析柱(200 mm×4.6 mm,5μm);流动相:乙腈-水,梯度洗脱;流速:1.0 ml·min~(-1);检测波长203 nm。结果:三七皂苷R_1、人参皂苷R_(gl)和R_(b1)分别在1.06～18.55μg(r=0.997 3)、2.02～35.35μg(r=0.998 2)和2.02～35.35μg(r=0.998 2)之间线性关系良好。平均回收率三七皂苷R_1为100.8%(RSD=1.53%,n=5),人参皂苷R_1为98.9%(RSD=1.87%,n=5),人参皂苷R_(b1)为99.7%(RSD=1.90%,n=5)。结论:HPLC梯度洗脱法能够将多种皂苷很好地分离检测,该方法准确可靠,重复性好,可用于控制其质量。
OBJECTIVE: To establish an HPLC gradient method for the simultaneous determination of three saponins R 1, R g 1 and R g 1 in Panax notoginseng capsule. METHODS: The analytical column of Spherisorb C_ (18) in Dalian (200 mm × 4.6 mm, 5 μm) was obtained. The mobile phase consisted of acetonitrile and water with gradient elution. The flow rate was 1.0 ml · min -1 and the detection wavelength was 203 nm . Results: The contents of notoginsenoside R_1, ginsenoside R_ (gl) and R_ (b1) were 1.06 ~ 18.55μg (r = 0.9973), 2.02 ~ 35.35μg (r = 0.9982) and 2.02 ~ 35.35μg 2) between the good linear relationship. The average recoveries of notoginsenoside R_1 were 100.8% (RSD = 1.53%, n = 5), ginsenoside R_1 was 98.9% (RSD = 1.87%, n = 5) and ginsenoside R b1 was 99.7% 1.90%, n = 5). Conclusion: HPLC gradient elution method can be a variety of well-separated saponin detection, the method is accurate and reliable, reproducible, and can be used to control its quality.