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目的:研究急性冠状动脉综合征(ACS)患者Th3细胞与斑块不稳定和急性冠脉动脉综合征的关系.方法:将91例患者分为4组:急性心肌梗死(AMI)组26例,不稳定型心绞痛(UA)组25例,稳定型心绞痛SA组22例和胸部疼痛综合征(CPS)组18例.采用流式细胞仪分析Th3细胞占CD4+T细胞的比例.应用酶联免疫吸附方法(ELISA)检测患者外周血血浆Th3相关细胞因子的表达.结果:与SA组和CPS组患者相比,ACS患者外周血Th3数量和TGF-1β1水平显著降低(P<0.01).AMI组患者(2.7±1.1)%及UA(2.5±0.9)%Th3细胞(CD4+TGF-1β1+/CD4+T细胞)比例明显低于SA组(4.94±1.7)%和CPS组(4.5±1.8)%,(P<0.01),而SA组和CPS组无明显差异(P>0.05).AMI组(4.84±2.64μg/L)及UA组(4.39±2.38μg/L)的TGF-1β1的浓度均显著低于SA组(7.40±4.22μg/L)和CPS组(6.57±3.76μg/L).结论:Th3细胞下调可能参与动脉粥样斑块不稳定和急性冠状动脉综合征的发病.
Objective: To study the relationship between Th3 cells and plaque instability and acute coronary syndrome in patients with acute coronary syndrome (ACS) .Methods: Ninety-one patients were divided into four groups: 26 patients in acute myocardial infarction (AMI) group, 25 cases of unstable angina pectoris (UA), 22 cases of stable angina pectoris (SA) group and 18 cases of thoracic pain syndrome (CPS) group.The proportion of Th3 cells in CD4 + T cells was analyzed by flow cytometry.Using enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of Th3 related cytokines in peripheral blood of patients with ACS.Results Compared with SA group and CPS group, the levels of Th3 and the level of TGF-β1 in peripheral blood were significantly decreased in ACS patients (P <0.01) The proportion of patients with (2.7 ± 1.1)% UA (2.5 ± 0.9)% Th3 cells (CD4 + TGF-1β1 + / CD4 + T cells) was significantly lower than that of SA patients (4.94 ± 1.7%) and CPS patients (4.5 ± 1.8% (P <0.01), but there was no significant difference between SA group and CPS group (P> 0.05), the levels of TGF-β1 in AMI group (4.84 ± 2.64μg / L) and UA group (4.39 ± 2.38μg / L) (7.40 ± 4.22μg / L) and CPS group (6.57 ± 3.76μg / L) .Conclusion: Down-regulation of Th3 cells may be involved in the pathogenesis of atherosclerotic plaque instability and acute coronary syndrome.