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观察滇产粗根荨麻水提取部分对佐剂性关节炎(adjuvant arthritis,AA)大鼠腹腔巨噬细胞(peritonealmacrophages,PMφ)分泌肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)及前列腺素E2(prostaglandin E2,PGE2)的影响。建立大鼠佐剂性关节炎模型,Ur水提取部分连续灌胃给药14或21 d后分次获取大鼠腹腔巨噬细胞,脂多糖(lipopolysaccharide,LPS)诱导大鼠腹腔巨噬细胞,用酶联免疫吸附法检测培养上清液中TNF-α及PGE2水平。AA大鼠腹腔巨噬细胞TNF-α及PGE2分泌较正常组升高,Ur水提取部分(400,200 mg/kg)对LPS诱导的AA大鼠腹腔巨噬细胞分泌TNF-α及PGE2水平有明显抑制作用。滇产粗根荨麻水提取部分对佐剂性关节炎的治疗作用可能与其抑制腹腔巨噬细胞分泌TNF-α及PGE2有关。
Observation on the secretion of tumor necrosis factor-α (TNF-α) by peritoneal macrophages (PMφ) in rats with adjuvant-induced arthritis (AA) And the effects of prostaglandin E2 (PGE2). A rat model of adjuvant arthritis was established. Rats peritoneal macrophages and lipopolysaccharide (LPS)-induced rat peritoneal macrophages were harvested at a time after 14 or 21 days of continuous administration of Ur water extract. Enzyme-linked immunosorbent assay was used to detect the level of TNF-α and PGE2 in culture supernatant. The secretion of TNF-α and PGE2 in peritoneal macrophages of AA rats was higher than that in the normal group. Ur water extract (400, 200 mg/kg) significantly inhibited the secretion of TNF-α and PGE2 from LPS-induced peritoneal macrophages in AA rats. effect. The therapeutic effect of water extraction fraction of R. communis on adjuvant arthritis may be related to its inhibition of TNF-α and PGE2 secretion from peritoneal macrophages.