【摘 要】
：
Rice stripe virus(RSV)is the causative agent of rice stripe disease and is completely dependent on insect vectors for its plant-to-plant transmission.Laodelphax
【机 构】
：
State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Science,Beijing,
论文部分内容阅读
Rice stripe virus(RSV)is the causative agent of rice stripe disease and is completely dependent on insect vectors for its plant-to-plant transmission.Laodelphax striatellus is the major insect vector for RSV In this study,we explored the interactions be-tween RSV infection and L.striatellus autophagy,a potential intrinsic antiviral mechanism in insects.We found that L.striatellus autophagic activity did not affect RSV infection;however,the autophagy-related-8(Atg8)gene significantly enhanced virus infection.Dur-ing RSV initial infection within the L.striatellus midgut,silencing of Atg8 expression significantly decreased the phosphorylation of c-Jun N-terminal kinase(p-JNK);however,when RSV infection is absent,silencing of Atg8 did not alter p-JNK levels.These results indicated that Atg8 might activate the JNK machinery by allowing more virus infection into cells.We further revealed that Atg8-deficiency significantly decreased RSV accumu-lation on the surface of the insect midgut epithelial cells,suggesting a receptor trafficking function of the γ-aminobutyric acid receptor-associated protein family.Using the RSV ovary entry as a model,in which vitellogenin receptor(VgR)mediates RSV cell entry,we clarified that Atg8-deficiency decreased the abundance of VgR localizing on the cytomem-brane and disturbed the attachment of RSV in the germarium zones.Collectively,these results revealed an autophagy-independent function of L.striatellus Atg8 that enhances RSV initial infection by increasing virus attachment on the infection sites.
其他文献
【摘要】目的：探讨一种新型EMS智能腰围在腰腹部肌肉损伤中的康复应用。方法：选择2020年2月至2020年7月治疗的126例腰腹部肌肉损伤患者，随机均分为对照组与试验组各63例，对照组患者给予常规热红外照射加普通腰围治疗，试验组患者应用一种新型EMS智能腰围治疗，两组患者均连续治疗21 d。结果：两组治疗7 d、21 d后视觉模拟评分、腰椎Oswestry功能障碍指数评分均显著低于治疗前，日本骨科
The Southern house mosquito Culex quinquefasciatus relies on its olfactory system to locate the human hosts for blood meals,by which several deadly diseases are
【摘要】目的：探讨分析为输卵管堵塞患者展开治疗的方法和临床效果，初步讨论揭示输卵管堵塞疾病与子宫内膜异位症的关系。方法：2018年2月至2020年4月将我院妇产科收治的104例输卵管堵塞患者随机等分为两组，每组各52例，参照组行宫腔镜手术治疗，研究组实施宫腔镜手术联合中药治疗，对比两组患者治疗效果满意度、术后1年妊娠成功率、手术过程持续时间、术中累计出血量、术后排气实现时间，以及接受治疗前后SAS
Krüppel homolog 1(Kr-hl),a zinc finger transcription factor,is involved in the metamorphosis and adult reproduction of insects.However,the role of Kr-hl in rep
Abstact Bumblebees play an impurtant role in maintaining the balance of natural and agricultural ecosystems,and the characteristic gut microbiota of bumblebees
【摘要】目的：分析手术室专干组长质量微信群在手术室的管理应用。方法：选择2019年6月至2020年6月我院的8名手术室专干组长，按照随机数表法的方式将所有手术室专干组长分为对照组（4例，实施常规管理），观察组（4名，在常规管理基础上实施质量微信群管理），观察两组手术室专干组长的满意度、手术室质量以及职业倦怠感。结果：实施微信群管理的观察组手术室专干组长的满意度以及手术室质量均要高于实施常规管理的对
The brown planthopper is a notorious rice pest in many areas of Asia.The evolution of insecticide resistance in Nilaparvata lugens has become a serious problem
【摘要】目的：分析过敏性鼻炎发病的危险因素与护理干预措施。方法：选取2018年3月至2019年10月我院收治的过敏性鼻炎患者70例为观察组，同期体检健康患者70例为对照组，分析发病后各种危险因素，并提出有效护理措施。结果：对过敏性鼻炎主要影响因素为：饮茶、父母过敏史、食物过敏、父亲吸烟、尘螨IgE阳性，均为过敏性鼻炎危险因素，差异有统计学意义（P0.05。 1.2方法 患者入院之后统一进行体检、
【摘要】目的：探讨小切口阑尾切除术治疗急性阑尾炎的临床疗效。方法：采用随机数字表法将我院2018年1月至2019年1月收治的400例急性阑尾炎患者分为两组，每组各200例。观察组采用小切口阑尾切除术治疗，对照组采用常规阑尾切除术治疗，比较两组临床指标、满意度及并发症。结果：观察组术中出血量、下床活动时间、排气时间、切口长度、住院时间及手术时间均比对照组低，差异有统计学意义（P<0.05）；观察组治
Bacteria are ubiquitous inhabitants of animals.Hormaphidinae is a particular aphid group exhibiting very diverse life history traits.However,the microbiota in t