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Objective:In the present study we try to correlate between pathogenic intrinsic factor of Escherichia coli(E.coli) presented with different fimbria genotyes and biofilm formation with host immune factor entitled interleukin-6(IL-6) secretion as defense mechanism.Methods:A total of 91 pediatrics complaining of pyuria were included in the present study.In addition,20 healthy control children were included.Full microbiological study was performed for isolated E.coli.PapC alleles were studied by multiple alleles PCR and biofilm formation was studied.IL-6 was measured in urine.Results:IL-6 had statistically significant elevation in patients’urine compared to control.From biofilm study, it was found that 19 isolated E.coli had formed biofilm in vitro.Moreover,urine samples with positive biofilm formation of E.coli had statistically significant lower IL-6 secretion than those with negative E.coli for biofilms.The distribution of fimbria genotypes showed that the frequent genotype was for alleleⅠ(34.3%) followed by mixed allelesⅠandⅡ(24.1%).There was significant correlation between mixed alleles(Ⅰ&Ⅱ)and biofilm formation.Conclusion: The present study highlights the presence of significant strains of E.coli causing urinary tract infections capable of biofilm formation.Biofilm formation is associated with less innate immunity manifested by lower urinary IL- 6.The majority of isolates had fimbria genes.It appears that mixed allelesⅠandⅡhave prominent virulence effect with tendency for biofilm formation.
Objective: In the present study we try to correlate between pathogenic intrinsic factor of Escherichia coli (E. coli) presented with different fimbria genotyes and biofilm formation with host immune factor entitled interleukin-6 (IL-6) secretion as defense mechanism. Methods: A total of 91 pediatrics complaining of pyuria were included in the present study. In addition, 20 healthy control children were included. Full-scale biologic study was performed for isolated E. coli. PapC alleles were studied by multiple alleles PCR and biofilm formation was studied. IL-6 was measured in urine. Results: IL-6 had statistically significant elevation in patients’urine compared to control. Fc biofilm study, it was found that 19 isolated E. coli had formed biofilm in vitro. biofilm formation of significantly higher IL-6 secretion than those with negative E. coli for biofilms. distribution of fimbria genotypes showed that the frequent genotype was for alle There was significant correlation between mixed alleles (I & II) and biofilm formation. Conlusion: The present study highlights the presence of significant strains of E. coli causing urinary tract infections capable of biofilm formation.Biofilm formation is associated with less innate immunity manifested by lower urinary IL- 6.The majority of isolates had fimbria genes.It appears that mixed alleles I and IIhave prominent virulence effect with tendency for biofilm formation.