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目的:制备鞣酸高乌甲素化合物,考察该化合物的理化性质并进行评价。方法:利用鞣酸对生物碱的沉降作用制备鞣酸高乌甲素,采用差示扫描量热法、紫外光谱、傅里叶红外光谱对该化合物进行鉴定。通过紫外分光光度法确定高乌甲素和鞣酸的摩尔比,在模拟人工胃液、小肠和结肠液环境中测定化合物对等量蛋白质的沉降能力。在考察该化合物对血液凝结能力的基础上,建立体表创伤模型测定该化合物对Wistar大鼠的伤口愈合能力。结果:鞣酸与高乌甲素按摩尔比1∶1形成新化合物。在人工胃液、小肠和结肠液环境中,鞣酸高乌甲素化合物对等量蛋白的沉降量分别为0.63,0.46,0.92 mg。化合物组对伤口愈合能力极显著高于空白组,鞣酸阳性组也具有伤口愈合能力,但效果不及新化合物组。结论:高乌甲素与鞣酸通过氢键作用形成了新化合物。该药物在弱碱性环境中通过沉降血液中蛋白而达到凝集血液效果,可促进伤口愈合、止血、消炎,提高了药物的生物应用价值。
OBJECTIVE: To prepare lappaconitine tannin compound and study the physical and chemical properties of the compound. Methods: The tannic acid was separated by tannic acid on the alkaloids. The compounds were identified by differential scanning calorimetry, UV-vis and Fourier transform infrared spectroscopy. The molar ratio of lappaconitine to tannic acid was determined by UV spectrophotometry to determine the compound’s ability to precipitate equivalent amounts of protein in a simulated artificial gastric, intestinal, and colonic fluid environment. On the basis of examining the ability of the compound to coagulate blood, a wound healing model was established to determine the wound healing ability of the compound on Wistar rats. Results: Tannic acid and lappaconitine at a molar ratio of 1: 1 formed a new compound. In artificial gastric juice, small intestine and colon fluid environment, the amount of laccase compound tannin equivalent amount of sedimentation were 0.63,0.46,0.92 mg. The wound healing ability of the compound group was significantly higher than that of the blank group, tannic acid positive group also had wound healing ability, but the effect was less than the new compound group. Conclusion: Lappaconitine and tannin formed new compounds by hydrogen bonding. The medicine achieves the effect of agglutinating blood by sedimentation of blood proteins in a weakly alkaline environment, which can promote wound healing, hemostasis and anti-inflammation, and improve the biological application value of the medicament.