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目的 :研究豚鼠耳蜗微血管内皮细胞跨细胞电阻的动态变化及峰值大小 ,以及细胞单层对12 5I 牛血清白蛋白的通透性。方法 :①用小池技术建立起该内皮细胞通透性的体外模型 ;②研究该体外模型跨细胞电阻及对12 5I 牛血清白蛋白的通透性。结果 :①耳蜗内皮细胞在培养增殖过程中其跨细胞电阻呈动态变化过程 ,以 5×10 4/cm2 的细胞密度接种时 ,7d左右电阻到达峰值 ,其跨细胞电阻峰值大小为 (118.9± 18.5 )Ω/cm2 ;②体外模型中加入12 5I 牛血清白蛋白后 ,贝克 时间变化图呈抛物线型上升曲线 ,90min内几乎呈直线。结论 :跨细胞电阻及对12 5I 牛血清白蛋白变化曲线反映了体外耳蜗微血管内皮细胞的通透性特征。
OBJECTIVE: To investigate the dynamic changes and the peak value of transcellular resistance of cochlear microvascular endothelial cells of guinea pigs and the permeability of 125 I Bovine Serum Albumin (BSA) in monolayers. Methods: ① The in vitro model of endothelial cell permeability was established by Koike technique; ② The cross-cell resistance of the in vitro model and the permeability of 125I Bovine serum albumin were studied. Results: ①Cross-cell resistance of cochlear endothelial cells changed dynamically during cell proliferation. When cells were seeded at a cell density of 5 × 10 4 / cm 2, the resistance reached the peak at 7 days, and the peak value of cross-cell resistance was (118.9 ± 18.5) ) Ω / cm2; ② After addition of 125I Bovine Serum Albumin (BSA) in vitro, the time course of Baker showed a parabolic curve, almost linear within 90min. CONCLUSIONS: The cross-cell resistance and the change of 125I Bovine serum albumin reflect the permeability characteristics of cochlear microvascular endothelial cells in vitro.