糖蛋白结合疫苗制备中赖氨酸单位减少率 (或赖氨酸单位替代率 )是衡量载体蛋白琥珀酰化程度的一个重要指标。本文建立了一种方便易行的化学检测方法 ,即氨基定量测定法 (简称改良Fields法 ) ,它是通过对琥珀酰化处理前和处理后载体蛋白中赖氨酸的ε一氨基的定量测定 ,间接计算出赖氨酸单位减少率大小的。经测试该方法中标准赖氨酸浓度的测试灵敏度范围在 0 .5～ 40 μg。同时用已知不同浓度赖氨酸和三批待测琥珀酰化载体蛋白进行验证 ,显示该方法具有良好的稳定性和重复性 Glycine protein conjugate vaccine preparation lysine unit reduction rate (or lysine unit replacement rate) is a measure of the degree of carrier protein succinylation an important indicator. This article established a convenient and convenient chemical detection method, namely amino quantitative determination method (referred to as the modified Fields method), which is based on the quantitative determination of lysine epsilon amino group of the carrier protein before and after succinylation treatment , Indirectly calculated lysine unit reduction rate. The test sensitivity of the standard lysine concentration tested in this method ranged from 0.5 to 40 μg. At the same time with known lysine lysine and three batches of tested succinylated carrier protein validation showed that the method has good stability and repeatability