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目的探讨丙泊酚对脂多糖(LPS)诱导的BV-2小胶质细胞活化的影响及其可能机制。方法体外培养小鼠BV-2小胶质细胞,随机分为LPS 1μg/ml组(A组)、丙泊酚30μM组(B组)、LPS 1μg/ml+丙泊酚30μM组(C组)和空白对照组(D组)。采用RT-PCR检测各组细胞中IL-1β和TNF-αmRNA表达量,Western blot检测总糖原合成酶激酶-3β(GSK-3β)和磷酸化GSK-3β(p-GSK-3β)的蛋白表达水平。结果 A、C组IL-1β、TNF-α及p-GSK-3表达量均较D组明显增加(P<0.05或P<0.01)。与A组相比,C组IL-1β和TNF-αmRNA表达量降低,而p-GSK-3β蛋白表达量增加(P<0.05)。结论丙泊酚30μM能在体外减轻LPS诱导的BV-2小胶质细胞释放IL-1β和TNF-α的水平,此作用可能与抑制GSK-3β活性有关。
Objective To investigate the effect of propofol on the activation of BV-2 microglia induced by lipopolysaccharide (LPS) and its possible mechanism. Methods BV-2 microglial cells were cultured in vitro and were randomly divided into 3 groups: group A (LPS 1μg / ml), group B (30μM propofol), group C Blank control group (D group). The expression of IL-1β and TNF-αmRNA in each group was detected by RT-PCR. The protein of total glycogen synthase kinase-3β (GSK-3β) and phosphorylated GSK-3β (p-GSK-3β) The expression level. Results The expressions of IL-1β, TNF-α and p-GSK-3 in group A and group C were significantly higher than those in group D (P <0.05 or P <0.01). Compared with group A, the expression of IL-1β and TNF-αmRNA decreased and the expression of p-GSK-3β increased in group C (P <0.05). Conclusion Propofol 30μM can reduce the level of IL-1β and TNF-α released by LPS-induced BV-2 microglia in vitro, which may be related to the inhibition of GSK-3β activity.