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作者测定了8名置Cu-IUD有正常月经周期妇女宫内膜的雌二醇-17β-脱氢酶(E_2DH)活性,研究对象年龄为19～37岁,置Cu-IUD8～33个月,刮宫术大部分在黄体中期进行,有2个标本则在增殖期取材,所有标本除部分作常规切片供组织学计算日期,其余冰冻保存,按以前方法测定E_2DH活性,其活性以每小时每毫克蛋白形成的雌酮毫微克分子数(E_1n mol/mg蛋白/h)表示。并同时确定Cu~(++)浓度对内膜E_2DH活性的影响。结果表明,置Cu-IUD妇女分泌期内膜的E_2-DH活性为13±8E_1n mol/mg蛋白/h(7～27n
The authors measured the activity of estradiol-17β-dehydrogenase (E_2DH) in 8 endometrial tissues of women with Cu-IUD normal menstrual cycle. The subjects were aged 19-37 years with Cu-IUD 8-33 months, Most of the curets were performed in the middle of the corpus luteum. Two specimens were obtained during the proliferative phase. All specimens were routinely sectioned for histological calculation and the rest were cryopreserved and assayed for E_2DH activity as previously described, Protein formation of estrone nanogram molecules (E_1n mol / mg protein / h) said. Meanwhile, the effect of Cu ~ (++) concentration on the activity of E_2DH in the intima was also determined. The results showed that the E_2-DH activity of endometrium secreting Cu-IUD was 13 ± 8E_1n mol / mg protein / h (7 ~ 27n