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为实现对特定生物分子的高灵敏度快速检测与分析,采用上转换发光材料作为标记物,研制成功一台基于上转换发光技术的新型光学免疫生物传感器。该传感器利用上转换发光材料在红外光激发下发射可见磷光的特性,通过对免疫层析试纸条上经生物反应而结合上去的上转换发光材料颗粒的含量进行检测,计算出被测样品中特定生物分子的浓度。实验结果表明,该传感器具有较好的生物特异性,对兔抗鼠疫免疫球蛋白(IgG)标准样品的检测灵敏度达到ng/ml量级,并在200～6000ng/ml浓度范围内具有良好的线性响应特性,相关系数R2≥0.95;对鼠疫耶尔森氏菌抗体的敏感性明显高于间接血凝实验,且与免疫印迹检测实验结果具有较好的一致性。该传感器具有稳定、可靠、灵敏的工作性能,符合实际检测与分析的要求。
In order to achieve high sensitivity and rapid detection and analysis of specific biomolecules, an up-conversion luminescent material was used as a marker to develop a new optical immunobiosensor based on up-conversion luminescence technology. The sensor utilizes the characteristic that the up-converting luminescent material emits visible phosphorescence under the excitation of infrared light, and detects the content of upconverting luminescent material particles bound by the biological reaction on the immunochromatographic test strip to calculate the content of the up- Specific biomolecule concentration. The experimental results show that the sensor has good biological specificity, the detection sensitivity of rabbit anti-plague immunoglobulin (IgG) standard sample reaches the level of ng / ml, and has good linearity in the range of 200-6000 ng / ml The correlation coefficient R2≥0.95. The sensitivity to Yersinia pestis was significantly higher than that of indirect hemagglutination, and it was in good agreement with the results of western blotting. The sensor has a stable, reliable and sensitive performance, in line with the actual detection and analysis requirements.