论文部分内容阅读
目的探讨P53的调控分子Apak亚细胞定位的结构基础。方法在热休克及DNA损伤诱导剂MMS处理下,通过免疫荧光技术观察Apak的亚细胞定位变化,并通过激光共聚焦荧光显微镜观察Apak截短体的亚细胞定位。结果 Apak多以均匀的形式分布在细胞核质,偶见在核仁中聚集;在外界刺激后Apak核内的均匀分布减少,主要以点状的大颗粒形式在核仁中呈聚集样分布;Apak的9种截短体均可定位于核内,且在核质和核仁中具有明显的比例差异。结论在DNA损伤信号下Apak有一个从核质向核仁移位的动态过程;KRAB区可以拮抗Apak在核仁的定位,而锌指尤其是锌指数的数目(锌指数>14)则决定了Apak能否在核仁定位。
Objective To investigate the structural basis of P53 subcellular localization of regulatory molecule Apak. Methods The subcellular localization of Apak was observed by immunofluorescence with heat shock and DNA damage inducer MMS. The subcellular localization of Apak truncated was observed by laser confocal fluorescence microscopy. Results Apak mostly distributed in the cytoplasm of the cytoplasm in even form and occasionally gathered in the nucleolus. Apak nucleus was uniformly distributed in the nucleus after external stimulation. Apak mainly distributed in the nucleolus in a spot-like form. Apak The 9 kinds of truncated bodies can be located in the nucleus, and the nucleoli and nucleoli in a significant proportion of differences. CONCLUSION Apak has a dynamic process of nuclear translocation from nucleus to nucleolus under the DNA damage signal. The KRAB region can antagonize the localization of Apak in the nucleolus, while the number of zinc fingers, especially the zinc index (zinc index> 14) Apak can be located in the nucleolus.