利用重组DNA技术,外源基因在不同的宿主系统中表达产生大量有医疗价值的人蛋白质已成为可能,大多数有临床意义的蛋白是糖蛋白,所以,它们的生物工程制备需要哺乳动物细胞为宿主,以产生具有正确翻译后加工的蛋白质。蛋白质上的糖的作用可从以下几方面加以讨论,其中包括:①糖对生物活性和在体内半寿期的影响;②糖与细胞膜上的专一受体的相互作用(对专一导向到不同的细 Using recombinant DNA technology, it has become possible for exogenous genes to be expressed in a variety of host systems to produce large numbers of human proteins of medical interest. Most of the clinically significant proteins are glycoproteins, so their bioengineering requires that mammalian cells be Host to produce the protein with the correct post-translational processing. The role of sugars on proteins can be discussed in several ways, including: (1) the effect of sugars on biological activity and in vivo half-life; (2) the interaction of sugars with specific receptors on the cell membrane Different details