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目的：探讨Ｗａｓｔｅｒｎｂｌｏｔ法对重症肌无力合并胸腺瘤（ＭＧＴ）患者的血清学诊断价值。方法：分别用蛋白印迹法和酶联免疫法检测３３例重症肌无力患者（有瘤１６例，无瘤１７例）、正常人１０例、其他疾病对照１８例血清中的人骨骼肌柠檬酸提取物抗体（ＣＡＥ－Ａｂ）。结果：ＣＡＥ抗原中３．４万，５．０万，６．０万组分与ＭＧＴ患者血清结合显色率分别为９３．７％（１５／１６例），７５．０％（１２／１６例），７５．０％（１２／１６例），而其他组无１例结合显色。蛋白印迹法、酶联免疫法对ＭＧＴ患者检出阳性率分别为１６／１６，１２／１６，假阳性率分别为０／１７，１／１７。结论：蛋白印迹法检测血清中ＣＡＥ－Ａｂ比酶联免疫法更敏感，特异性高。但蛋白印迹法比酶联免疫法操作复杂，费用较高。
Objective: To investigate the serological diagnostic value of Wasternblot in patients with myasthenia gravis and thymoma (MGT). Methods: Western blotting and enzyme-linked immunosorbent assay were used to detect 33 cases of myasthenia gravis (16 cases with tumor, 17 cases without tumor), 10 cases with normal controls and 18 cases with other disease controls Antibody (CAE-Ab). Results: The colorimetric rates of 34,000, 50,000 and 60,000 of CAE antigens were 93.7% (15/16), 75.0% (12/16 Cases), 75.0% (12/16 cases), while no other group combined with color development. Western blotting and enzyme-linked immunosorbent assay showed that the positive rates of MGT were 16/16, 12/16 respectively and the false-positive rates were 0/17 and 1/17 respectively. CONCLUSION: Western blotting of serum CAE-Ab is more sensitive and specific than enzyme-linked immunosorbent assay. However, Western blotting is more complex and costly than enzyme-linked immunosorbent assay.