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提高PCR-STR分型技术的准确性。采用荧光标记dNTP掺入法,通过DNA自动测序仪分析不同等位基因片段的长度并确定其命名后,建立并比较扩增产物混合-纯化-重扩增法、琼脂糖凝胶电泳-切割回收纯化DNA-重扩增法、非变性聚丙烯酰胺凝胶电泳-切割回收纯化DNA-重扩增法等3种Ladder制备方法。扩增产物混合-纯化-重扩增法相对较简便、快速、经济。Ladder对照使基因分型数据化,判型准确,重复性好,有利于标准化的实现。
Improve the accuracy of PCR-STR typing. Using fluorescent labeled dNTP incorporation method, the lengths of different alleles were analyzed by DNA sequencer and their nomenclature was established. Then, the amplification products were mixed and purified, re-amplified, and separated by agarose gel electrophoresis. Purification of DNA - re-amplification, non-denaturing polyacrylamide gel electrophoresis - DNA purification and re-amplification amplified three kinds of Ladder preparation method. Amplification product mix - purification - re-amplification method is relatively simple, fast and economical. Ladder control genotyping data, accurate sentence classification, good repeatability, is conducive to standardization.