为了合理选择细胞毒药物治疗转移癌,使用 Hamburger 和 Salmon 的软琼脂克隆形成法,检测周期长达2～3周,生长率低,死细胞造成假团块状物,出现5～10%的假阴性。为解决这些问题,在上述测试方法基础上,作者又发展了~3H 胸腺嘧啶掺入作为肿瘤生长终点的快速检测法。使用此法,5天即可测得结果,并可消除死细胞团块状物造成的假阴性。材料与方法 In order to rationally select cytotoxic drugs for the treatment of metastatic cancers, the soft agar colony formation assay of Hamburger and Salmon was used with a detection period of up to 2 to 3 weeks, a low growth rate, dead clumps causing clumps and 5 to 10% negative. In order to solve these problems, based on the above test methods, the authors developed ~ 3H thymidine incorporation as a rapid assay for tumor growth. Using this method, the results can be measured in 5 days and the false negatives caused by dead cell clumps can be eliminated. Materials and Methods