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背景 肿瘤术中血液回收因为血液污染有肿瘤细胞和全身扩散而被禁用。那些对照射敏感的肿瘤细胞,给与回收血50Gy照射用于解决肿瘤手术回收血的再利用问题。研究设计与方法作者用10个细胞系和14个加入大剂量肿瘤细胞的细胞制剂,或肿瘤外科手术时收集的血液做体外研究照射去除肿瘤细胞的能力。γ-照射前后,肿瘤细胞经密度梯度分离,用细胞集落形成检测其增殖能力。结合5’-溴去氧尿苷分析DNA的代谢。结果 不同肿瘤细胞的存活曲线证实,DO值(减少存活细胞至
Background Tumor surgery Blood is banned because of blood contamination of tumor cells and systemic spread. Those irradiation-sensitive tumor cells, given the recovery of blood 50Gy irradiation is used to solve the problem of reusing the blood recovered by tumor surgery. Study Design and Methods The authors performed in vitro studies on the ability of tumor cells to be irradiated with 10 cell lines and 14 cell preparations with high dose of tumor cells or blood collected during tumor surgery. Before and after γ-irradiation, the tumor cells were separated by density gradient, and the proliferation of tumor cells was detected by colony formation. DNA metabolism is analyzed in conjunction with 5’-bromodeoxyuridine. Results The survival curves of different tumor cells confirmed that DO values (decreased viable cells to