评价钙拮抗药对血管平滑肌的作用,对于了解其治疗作用的性质有重要意义。遗憾的是,因所使用的方法(包括血管类别及动物种属)不同,明显影响实验结果。本研究目的在于观察改变同一种标本的实验条件(如溶液、实验方案等)对药物钙拮抗活性的影响。作者比较了三种通常用以研究钙拮抗药的方法,因为钙拮抗药对KCl引起兔主动脉环的除极化性收缩非常敏感,三种实验均采用KCl引起动脉环收缩。以双氢吡啶衍化物PY 105-068(PY)及戊脉安下简称(V)作为钙拮抗药,溶剂为对照。处死动物后,切割一段降主动脉,然后再切成宽2～3cm的动脉环,悬吊于含Tris缓冲液或 Evaluating the effect of calcium antagonists on vascular smooth muscle plays an important role in understanding the therapeutic properties of calcium antagonists. Unfortunately, the experimental results are significantly affected by the different methods used, including vessel type and animal species. The purpose of this study is to observe the effect of changing experimental conditions (such as solution, experimental protocol, etc.) on the calcium antagonistic activity of the same specimen. The authors compared three methods commonly used to study calcium antagonists because calcium antagonists were very sensitive to the depolarization of rabbit aortic rings induced by KCl, and KCl was used in all three experiments to cause contractions of the arterial rings. Dihydropyridine derivatives PY 105-068 (PY) and verapamil (V) as calcium antagonists, solvent control. After the animals were sacrificed, a section of the descending aorta was cut, and then cut into 2 to 3 cm wide arterial rings, suspended in Tris buffer