论文部分内容阅读
目的 探讨肝纤维化大鼠胶原网络的三维形态构筑的变化。 方法 采用健康雄性Wistar大鼠 ,随机分为对照组和肝纤维化组 ,后者采用 6 0 %的四氯化碳植物油皮下注射 ,造成肝纤维化大鼠模型 ,分别于实验的第10周和 12周末取材 ,作为 10周和 12周肝纤维化组 ,行天狼猩红显色 ,光镜下观察和氢氧化钠浸渍 ,扫描电镜下观察胶原网络的构筑。 结果 扫描电镜下 ,随着肝纤维化程度的加重 ,增生的胶原纤维交织成网 ,分隔包裹正常肝组织 ,形成大小不等圆形或椭圆型肝组织团块 ,近似于“蜂窝状”囊性的假小叶结构 ;相邻假小叶之间的胶原纤维沉积明显 ,并与包裹门管区和血管的纤维鞘相延续为间隔。与对照组相比 ,10周肝纤维化组形成了尚不完整的假小叶囊 ,12周组的小叶囊致密完整。 结论 肝纤维化时胶原纤维超微构筑发生了改变 ,增生的胶原网络主要由粗、细纤维构成。
Objective To investigate the changes of three-dimensional morphological architecture of collagen network in rats with hepatic fibrosis. Methods Healthy male Wistar rats were randomly divided into control group and hepatic fibrosis group. The latter group was subcutaneously injected with 60% carbon tetrachloride vegetable oil to induce hepatic fibrosis in rats. At the tenth week and Twelve weeks after the harvest, as 10 weeks and 12 weeks of liver fibrosis group, line Sirius red color, light microscopy and sodium hydroxide impregnation, scanning electron microscopy collagen network construction. Results Under the scanning electron microscope, as the degree of hepatic fibrosis increased, the proliferating collagen fibers interweaved into meshes and separated the normal liver tissue and formed round or oval liver mass with the sizes ranging from “honeycomb” cysticity Of the false leaflet structure; between the adjacent pseudolobules collagen deposition was obvious, and with the parietal region and the vascular sheath of the fiber continued into the interval. Compared with the control group, the 10-week liver fibrosis group formed an incomplete pseudolobule capsule, and the 12-week group had a dense and complete leaflet capsule. Conclusion The ultrastructure of collagen fibers changes in liver fibrosis. The collagen network of hyperplasia mainly consists of coarse and fine fibers.