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目的:建立防己薏连丸的质量标准。方法:采用显微鉴别法鉴别苦杏仁、地黄、炒僵蚕;采用薄层色谱法鉴别薏苡仁、连翘、忍冬藤;采用高效液相色谱法测定粉防己碱、防己诺林碱以及连翘苷的含量。结果:显微鉴别法专属性强;薄层色谱法色谱特征明显,斑点清晰。分离度好;高效液相色谱法测定含量,粉防己碱、防己诺林碱、连翘苷分别在0.125 0~1.999 8μg(r=0.999 8)、0.062 4~0.997 9μg(r=0.999 8)、0.100 2~1.602 6μg(r=0.999 7)范围内有良好的线性关系,平均回收率分别为98.87%(RSD=0.71%,n=6)、100.19%(RSD=1.70%,n=6)、97.79%(RSD=1.72%,n=6)。结论:所建立的试验方法操作方便、结果准确、专属性强,可用于防已薏连丸的质量控制。
Objective: To establish the quality standard of anti-Yi-Lian-Pill. Methods: Identification of bitter almond, Rehmannia glutinosa and Stir-fried silkworm by microscopic identification; Coix seed, Forsythia suspense and honeysuckle were identified by TLC, and tetrandrine, Glycoside content. Results: The micro-identification method is more specific; TLC has obvious chromatographic features and clear spots. The content of tetrandrine, forsythromycin and forsythin in the range of 0.125 0-1.9999 μg (r = 0.999 8), 0.062 4-0.9997 9 μg (r = 0.999 8) The average recoveries were 98.87% (RSD = 0.71%, n = 6), 100.19% (RSD = 1.70%, n = 6) in the range of 0.100 2 ~ 1.602 6μg (r = 0.999 7) 97.79% (RSD = 1.72%, n = 6). Conclusion: The established test method is convenient, accurate and specific. It can be used for quality control of anti-Yi-lian Pill.