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采用免疫细胞化学双 PAP法 ,观察雌二醇 (E2 )、孕酮 (P)对贝美格 (Bemegride,Be)腹腔致痫大鼠顶叶大脑皮层、海马 CA1、 CA3区 Glu和 GABA免疫反应细胞的影响。图像分析结果显示 :Be致痫组皮层、海马 Glu免疫反应平均阳性细胞数及光密度较正常组明显增加 (P<0 .0 1) ;GABA细胞数及光密度减少 (P<0 .0 1)。给予 E2 后 ,Be致痫大鼠大脑皮层、海马 Glu阳性细胞数目增多 ,光密度增高 (P <0 .0 1) ,GABA阳性细胞数目减少、光密度降低 (P <0 .0 5 ,P <0 .0 1) ;而给予 P后 ,致痫组 GABA阳性细胞数目增多、光密度增高 (P<0 .0 1) ,Glu阳性细胞数目减少、光密度减低 (P<0 .0 1)。提示雌、孕激素的致痫、抗痫作用与其调节脑内 GABA和 Glu系统的兴奋性有关。
The immunocytochemical double-PAP method was used to observe the effects of estradiol (E2) and progesterone (P) on Glu and GABA immunoreactivity in the parietal cortex, hippocampal CA1 and CA3 areas of rats with epilepsy induced by Bemegride (Be) Effects of cells. The results of image analysis showed that the mean number of positive cells and the optical density of Glu immunoreactivity in cortex and hippocampus of Be epileptogenic group were significantly higher than those in normal group (P <0.01); the number of GABA cells and optical density were decreased (P <0.01) ). After administration of E2, the number of Glu-positive cells in hippocampus and hippocampus of Be-induced epileptic rats increased, the optical density increased (P <0.01), the number of GABA positive cells decreased and the optical density decreased (P <0.05, P < The number of GABA positive cells increased, the optical density increased (P <0.01), the number of Glu positive cells decreased and the optical density decreased (P <0.01). Tip estrogen and progesterone induced epilepsy, anti-epileptic effect and its regulation of brain GABA and Glu system excitability related.