用抗补体免疫荧光染色在111份原发性肝癌患者血清中发现8份血清为抗乙肝病毒相关核抗原(HBNA)阳性,阳性率为7.2％。与国外相比,我国肝癌患者中抗HBNA阳性率略高,可能与乙肝病毒(HBV)感染有关,所有抗HBNA阳性血清均为抗HBC阳性,而HBV DNA均为阴性,提示HBNA在HBV停止复制后出现。用免疫荧光法,所有的抗核抗体(ANA)阳性血清均使PLC/PRF/5细胞着色,但所有的抗HBNA阳性血清均不使大鼠肝细胞着色。此结果表明抗HBNA与ANA无关。抗HBNA与HBNA是一对新的抗原抗体。 Eight serum samples from 111 primary hepatocellular carcinoma patients were found to be positive for anti-hepatitis B virus associated antigen (HBNA) with anti-complement immunofluorescence staining, with a positive rate of 7.2%. Compared with foreign countries, the positive rate of anti-HBNA in patients with hepatocellular carcinoma in our country is slightly higher, which may be related to hepatitis B virus (HBV) infection. All anti-HBNA positive sera are anti-HBC positive and HBV DNA are negative, After appearing. Using immunofluorescence, all of the ANA positive sera stains PLC / PRF / 5 cells, but none of the anti-HBNA positive sera stain the rat hepatocytes. This result indicates that anti-HBNA has nothing to do with ANA. Anti-HBNA and HBNA are a pair of new antigen antibodies.